- Zinc aluminum ion identification fluorescence probe as well as preparation method and application thereof
The invention provides a zinc aluminum ion identification fluorescent probe and a preparation method and application thereof, wherein a Schiff base structure and three benzyl groups are contained in the fluorescent probe molecule. The zinc-aluminum ion fluorescent probe provided by the invention has a double mechanism. The method has the characteristics of double response, high sensitivity, high specificity, no influence on other metal ions, easy cell uptake, low cytotoxicity and the like. The probe is applied to peripheral blood of prostate cancer patients, can quantitatively detect the zinc content in serum, and provides valuable information for diagnosis of suspected prostate cancer patients. The prostate cancer serum PSA is detected and combined, the diagnosis accuracy of prostate cancer can be effectively improved, the puncture diagnosis requirement of patients in the grayscale range of the serum PSA is avoided, and further excessive diagnosis and excessive treatment can be avoided.
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Paragraph 0033; 0035; 0036
(2021/09/21)
- Antiproliferative and apoptosis-inducing activities of alkyl gallate and gallamide derivatives related to (-)-epigallocatechin gallate
Green tea and (-)-epigallocatechin gallate (EGCG: one of the main components of green tea) are reported to have cancer-preventive activity in humans. A previous SAR study of EGCG and derivatives indicated that a galloyl group is essential for the activity. To test this hypothesis, we synthesized various alkyl gallate and gallamide derivatives and evaluated their antiproliferative effects on human leukemia HL-60 cells. Dodecyl 3,4,5-trihydroxybenzoate (6c) showed the most potent activity, being more potent than EGCG. To clarify the molecular mechanism of the antiproliferative action, we investigated the effects of 6c on various factors. Compound 6c was found to induce apoptosis mediated by endoplasmic reticulum (ER)-stress-related caspase-12. Upregulation of gadd-153, an ER-stress marker protein, was also observed. These results indicate that 6c induced apoptosis via the ER-stress-related pathway.
- Dodo, Kosuke,Minato, Taro,Noguchi-Yachide, Tomomi,Suganuma, Masami,Hashimoto, Yuichi
p. 7975 - 7982
(2008/12/23)